Two-time women's champion Elina Svitolina has crashed out of the Italian Open, while a knee injury has forced Serena Williams to pull out of the tournament.
Fifth seed Svitolina lost 6-4,1-6, 7-5 against Victoria Azarenka in the second round, while Serena announced her withdrawal just before her match against older sister Venus.
Czech fourth seed Karolína Plisková also advanced to the third round along with Australian eighth seed Ashleigh Barty.
In the men's competition, world no. 5 Alexander Zverev suffered a shock 7-5, 7-5 defeat to Italian wildcard Matteo Berrettini, while Belgian David Goffin rallied from a set down to beat Stan Wawrinka 4-6, 6-0, 6-2.
Ninth seed Marin Cilic, seventh seed Juan Martin Del Potro, Australian Nick Kyrgios and Portugal's Joao Sousa are also through to the second round.
India finished day three of the second test on 64-1 to lead England by 244 runs after they dismissed the hosts for 407 in the first innings at Edgbaston on Friday despite Jamie Smith's commanding and unbeaten knock of 184.
There were to be no Fourth of July celebrations for American Madison Keys as she joined the exodus of seeds from Wimbledon with a 6-3 6-3 defeat by 104th-ranked German Laura Siegemund in the third round on Friday.
Prime Minister Luis Montenegro joined members of Diogo Jota’s family for a private wake on Friday in the Liverpool footballer’s hometown in northern Portugal following his death alongside his brother Andre Silva in a car crash in Spain.
Catching a millionaire Grand Slam champion in action on the second-tier Challenger Circuit is a rare sight, especially since cheering fans and creature comforts are in short supply when compared to the glitz and glamour of the main ATP Tour events.